Evaluation of a novel TaqMan probe-based real-time polymerase chain reaction (PCR) assay for detection and quantitation of red sea bream iridovirus

Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction.

A simple and sensitive polymerase chain reaction (PCR) based assay is described for detection of the red sea bream iridovirus (RSIV) in infected fish. The assay involves amplification of a portion of the ribonucleotide reductase small subunit (RNRS) gene of the virus from DNA isolated from the spleen. The system was tested on red sea bream following an experimental infection. In our infection m...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

evaluation of the performance characteristics of an in-house one step taqman real time-polymerase chain reaction assay for detection and quantification of hepatitis c virus

conclusions the in-house 1 step taqman real time rt-pcr assay showed acceptable performance characteristics. our study presents the robustness and cost-effectiveness of the method for detection and quantification of hcv rna. methods the primers and probe were selected from a highly conserved region of the hcv genome, which allowed the detection of 4 common hcv genotypes in iran. using 4 quantif...

detection of medically important candida species by absolute quantitation real-time polymerase chain reaction

results all eight candida species were successfully detected, identified and quantitated based on the icl gene. a seven-log range of the gene copy number and a minimum detection limit of 103 copies were achieved. conclusions a one-tube absolute quantification real-time pcr that differentiates medically important candida species via individual unique melting temperature was achieved. analytical ...

A New TaqMan Real-Time Polymerase Chain Reaction Assay for Quantification of Fusarium virguliforme in Soil

Mbofung, G. C. Y., Fessehaie, A., Bhattacharyya, M. K., and Leandro, L. F. S. 2011. A new TaqMan real-time polymerase chain reaction assay for quantification of Fusarium virguliforme in soil. Plant Dis. 95:1420-1426. The quantification of the soilborne pathogen Fusarium virguliforme inoculum in soil is important for epidemiological studies of soybean sudden death syndrome (SDS). Classical dilut...